Protect your valuable research time and samples with a compact electroporation machine designed and engineered for performance, speed, simplicity, and flexibility. The combination of the Invitrogen Neon NxT Electroporation System's patented pipette tip technology, simplified workflow, and flexibility helps reduce sample loss, lowers the risk of contamination, saves you steps, and can put you in control of his experiments.
Benefits of the Neon NxT Electroporation System
- Proven transfection efficiency and cell viability—transfecting challenging cell lines with high efficiency and high cell viability
- Flexibility when you need it— Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols.
- Save valuable research time—transfect cells quickly with a simple three-step workflow, a single buffer kit, and an intuitive user interface
- Preserve your valuable samples—minimize lost sample transfer and risk of contamination
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Achieve high transfection efficiency and high cell viability in a wide range of cell lines using this transfection system
The Neon NxT Electroporation System is an electroporation instrument that offers transfection and gene editing efficiency of up to 90% in extremely difficult-to-transfect cells, including immune, primary, and stem cells. More than 150 cell lines have been tested with ready-to-use conditions optimized for efficiency and viability.
Search theTransfection protocols and appointmentsdatabase for optimized electroporation protocols in different cell lines.
Figure 1. Transfection efficiency with the Neon NxT electroporation system.Cells were transfected with GFP plasmid DNA or GFP mRNA in either 10 µL or 100 µL electroporation reactions. Transfection efficiency is reported as the percentage of GFP-positive cells (n = 3).Use:Naive T cells were only electroporated in 100 µL reactions.
Figure 2. Cell viability after electroporation in the Neon NxT system.Cells were transfected with GFP plasmid DNA or GFP mRNA in either 10 µL or 100 µL electroporation reactions. Transfected cells were stained with Invitrogen SYTO Red Dead Cell Stain and viability was assessed on the Invitrogen ATTUNE NxT flow cytometer. Cell viability (%) is reported as the mean of 3 measurements.Use:Naive T cells were only electroporated in 100 µL reactions.
Why is the Neon NxT Electroporation System a better alternative to the electroporation cuvette?
Figure 3. Advantages of the Neon NxT pipette tip compared to the standard electroporation cuvette.The patented biologically compatible(A)The Neon NxT tip features proven capillary electroporation technology that has significant advantages over(B)electroporation cuvettes.
Unlike standard cuvette-based electroporation chambers, the Neon NxT Electroporation System uses a patented, biologically compatible pipette-tip chamber that generates a more uniform electrical field. This design allows for better maintenance of physiological conditions, resulting in high cell survival compared to conventional electroporation.†
- Creates a more uniform electric field.
- Maintains a stable pH throughout the electroporation chamber
- Generates minimal heat
- form fewer ions
- Reduces the shear force experienced by cells.
Precisely optimize electroporation parameters for different cell types, cell densities, payloads, and electroporation protocols
Customizable electroporation parameters
With the Neon NxT Electroporation System, you can precisely control the parameters that are most important to your experiment without wasting precious time adjusting the ones that aren't. This transfection system allows you to modify:
- pulse voltage
- Pulse width
- number of pulses
- cell type
- buffer type
- payload type
Versatility for different payloads, cell types, and cell densities
Deliver DNA, RNA, or protein to a wide variety of mammalian cells, with the flexibility to transfect 2 x 10⁴ to 6 x 10⁶ cells per reaction.
Plan easily with the TransfectionLab cloud-based app
Invitrogen TransfectionLab, a cloud-based application located atThermo Fisher connection, you can automatically generate a step-by-step guide tailored to each electroporation experiment, starting from the design stage. You can remotely configure multiple plate layouts for up to 384 samples, and any protocol or plate layout saved in the app can be directly accessed on the connected Neon NxT system.
Quickly transfect cells with a simple three-step workflow, a single buffer kit, and an intuitive user interface
Simplified Electroporation Workflow
Easy to follow and execute, the streamlined workflow of the Neon NxT Electroporation System requires minimal training and improves consistency and reproducibility.
Using this transfection system, the electroporation process is simple: first, take the cells and mix the plasmid in the tip of the Neon NxT pipette. Second, connect it to the pipette holder and press Electroporate. Finally, pipet the transfected cells back into your culture vessel. Since transfection occurs at the pipette tip, transfection is completed in just 3 easy steps: aspirate, electroporate, and dispense.
| Paso 1 |
|---|
AspirePrepare cells by suspending them in Neon NxT buffer. Load cells and payload mix suspended inNeon NxT cap on the Neon NxT pipette tip. |
| Paso 2 |
electroporadoApply electrical pulses to the cells to deliver the payload into the specialized buffer. |
| Paso 3 |
DismissRestore growth conditions to allow the transfected cells to recover. |
| Stage 4 |
analyze cellsAssess: e.g. gene expression, genome editing, silencing and cell line development |
Less processing time with this electroporation system
In addition to simplifying workflow, our patented electroporation technology shortens the overall process to 10-15 minutes, compared to a conventional electroporation system, since transfection occurs in the Neon NxT pipette tip.†
A single buffer kit for many mammalian cell types
Take the hassle out of searching for a buffer kit that will work with your cell line. We have simplified the process with a buffer kit that is compatible with over 150 mammalian cell lines. We recommend using Invitrogen Neon NxT Resuspension Buffer R at voltages below 1900 V and Invitrogen Neon NxT Resuspension Buffer T at higher voltages.
Time-saving plate setup
Instead of adjusting electroporation parameters between samples with the Quick Start, set up an entire plate in advance using the Neon NxT Electroporation System's intuitive user interface. Monitor progress on the screen as you electroporate each of your samples and electroporate up to 24 samples in less than 20 minutes.
Minimize sample transfer loss and risk of valuable cell contamination with a unique and compact electroporation instrument design
Minimal sample transfer loss
Your samples are precious, but some volume is inevitably lost during transfer to and from a conventional electroporation cuvette. Electroporation in the Neon NxT Electroporation System occurs within the pipette tip, helping to minimize sample loss and the amount of shear force experienced by cells.
Minimal sample contamination
The compact size of the Neon NxT system, including the pulse generator and pipetting station, makes it small enough to fit in most biosafety cabinets (BSCs). Your samples always remain within the aseptic area while you are using the instrument, helping to reduce the risk of contaminating precious cells. The Neon NxT system also has a new cable management feature that makes it easy to use at the BSC.
Figure 4. Dimensions of the Neon NxT electroporation system.The Neon NxT Pulse Generator (9.5"W x 7.6"H x 9.9"D) and Neon NxT Pipetting Station (4.9"W x 11.5"H x 5.9 inches deep) fit inside most BSCs, helping to minimize sample contamination.
Specification Comparison Between the Neon NxT Electroporation System and the Neon Transfection System
| Specification |  Neon NxT electroporation system |  Neon Transfection System |
|---|---|---|
| Electroporation volume | 10 µL o 100 µL | 10 µL o 100 µL |
| Electroporation buffer volume* | 2ml | 3ml |
| tip accessory | ClipTip Technology | Friction |
| electroporation pulses | 1-10 | 1-10 |
| pulse duration | 1-100ms | 1-100ms |
| pulse voltage | 500-2500 V | 500-2500 V |
| arc detection | Yeah | No |
| cloud connectivity | Yeah | No |
| Pulse Generator Dimensions** | 9.5 x 7.6 x 9.9 inches (W x H x D) 11.9 pounds (5.4 kg) | 9.5 x 8.9 x 13.6 inches (W x H x D) 13.8 pounds (6.25 kg) |
| Cable management feature*** | Yeah | No |
| Touch screen | 8 inch capacitive touch screen | 7 inch touch screen |
| electrical rating | 100-240 VCA, 270 W | 100-240 C, 150 W |
*The Neon NxT Electroporation System buffer tube has a 2 mL level indicator.
**The Neon NxT Pulse Generator can be moved into or out of a typical BSC without removing the sash.
***Excess cable length can be secured behind the Neon NxT system with the attachable cable manager.
Same technology and performance as the neon transfection system
Figure 5. Performance of the Neon NxT electroporation system and the Neon transfection system.Performance was assessed by transfecting different mammalian cell lines with GFP plasmid DNA or GFP mRNA.(A)GFP plasmid DNA transfection efficiency reported as the percentage of GFP-positive cells.(B)Viability of cells after transfection with GFP plasmid DNA.(C)GFP mRNA transfection efficiency reported as the percentage of GFP-positive cells.(D)Cell viability after transfection with GFP mRNA.
† Kim JA, Cho K, Shin MS,et al. (2008)A new method of electroporation using a wire-type and capillary electrode..Biosens Bioelectron23(9):1353–1360.
Resources
Documents
- Quick Reference Guide: Neon NxT Electroporation System
- Spec Sheet: Neon NxT Electroporation System
- Infographic Brochure: Invitrogen Neon NxT Electroporation System
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